SYSTEMATICS
Class Polyplacophora Gray, 1821
Order Chitonida Thiele, 1909
Suborder Chitonina Thiele, 1909
Family Ischnochitonidae Dall, 1889
Genus Tripoplax Berry, 1919
Tripoplax cf. balaenophila (Schwabe & Sellanes, 2004)
Fig. 1
Material examined
TALUD XV, St. 23, 27°08′11″N, 114°32′54″W, August 1, 2012, 5 specimens (TL 6.82–13.76 mm), benthic sledge, 530–625 m depth (EMU-10816 and 10817).
Morphological remarks
Small-sized animal (TL, 13.76, 11.21, 10.22, 6.98 and 6.82 mm) (Fig. 1i, j). Color yellowish-beige, girdle of the same ground color, with slightly darker bands. Tegmentum. General valve sculpture consisting of very fine granules arranged in quincunx (Fig. 1a, c, e), readily visible on the head valve, lateral areas and postmucronal area. Articulamentum consisting of a thin translucent white layer. Insertion plates short, with broad, sharp teeth (Fig. 1b, f). Slit formula: 10/1-2/17 (vs. 10/1-2/12 in the holotype), slit rays present. Ctenidia (or gills) in a holobranchial arrangement, left and right series of ctenidia counting 17–18 (Fig. 1j). Perinotum. Girdle rather small, covered with larger rectangular scales (>100 μm), the later sculptured with about 10 radial ribs (Fig. 1g, h). Radula (Fig. 1k, l). Central tooth slender, narrower in its middle part, provided with a small, upper fold inclined downwards. First lateral tooth shorter than central; turbercule-like structure in the corner of the blade directed outward. Second lateral tooth with inner denticle sharp, nearly twice as long as outer one; outer denticles obtuse. Major uncinal tooth slender, a lateral fold at mid-third, tip flattened, round, paddle-like.
General features of the examined material fit well with the original description (Schwabe & Sellanes 2004). However, although quite similar, girdle scales are proportionally larger and more rectangular in the Mexican specimens. The central tooth of the radula shows a distinctly different shape compared to these tooth in the original description of T. balaenophila, i.e., rectangular with a forward and downward bent single blade. The first lateral tooth is shorter in relation to the central one in our specimen and the tubercule-like structure in the corner of the blade is directed outward vs. inward directed in T. balaenophila.
Photographs of one specimen (TL, 11.21 mm; Fig. 1i, j) of T. cf. balaenophila were sent to Boris Sirenko who suggested that it could belong to that species (B. Sirenko, pers. comm., January 2015). SEM photographs of the radula and the girdles scales were sent to Enrico Schwabe who emphasized the similarity between T. balaenophila and our material, but suggested that the variations observed in the radula and in the girdles scales might indicated that the material reported herein represents a species very close yet distinct to T. balaenophila (E. Schwabe, pers. comm., September 2015). The differences observed might be significant, but a more profound revision is needed, including comparing the material with other species in the context of a thorough revision of Tripoplax (and Lepidozona), both morphologically and using molecular markers.
Environmental conditions
The species was collected at 530–625 m depth in the following environmental conditions: dissolved oxygen, <0.25 ml/l; temperature, 6.44 °C; salinity, 34.47.
Type locality
Off Concepción, Chile, 36°29.9′S; 73°40.8′W (Fig. 2), in 240 m depth, just beneath the shelf break, mostly attached to bones of dead whales and rocks.
Distribution and habitat
The presence of T. balaenophila off western Mexico would considerably extends its geographic range by over 63° of latitude (ca 8280 km) to Baja California (27°08′11″N; 114°32′54″W) (Fig. 2), and its bathymetric range to 530–625 m. There was no evidence of osseous remains in the sample and we believe that the specimens were probably attached to the rocks found in the net, and that were accidently collected. There was no indication that the sample was obtained from a chemosynthetic environment, as hypothesized earlier (Schwabe & Sellanes 2004). It is clear, however, that our material was collected just below the Oxygen Minimum Zone (OMZ) in severe hypoxic conditions (<0.25 ml/l O2). The OMZ has long been recognized as an ecologically important feature of the eastern Pacific, including western Mexico (Diaz & Rosenberg 1995; Hendrickx & Serrano 2010; Serrano 2012). According to published information (Schwabe & Sellanes 2004; Sellanes et al. 2010), the specimens from Chile were collected in bottom water with severe oxygen deficiency (<0.5 ml/l). The amplitude of the OMZ corresponding to upper and lower oxygen concentrations of 0.5 ml/l is reduced and shallower off Concepción, in southern Chile (from 50 to 150 m depth) (Helly & Levin 2004) when compared to the conditions found at the Mexican sampling locality (TALUD XV, St. 23, from 90 to >630 m depth) (D. Serrano, pers. comm., April 2015). In this area (ca 27 °N), the OMZ fringe corresponding to concentrations of 0.5 ml/l extends from 200 to 750 m (Helly & Levin 2004), a similar range as what is shown by the TALUD XV cruise data. This probably explained why the Mexican material was collected in deeper water than off Chile. On the other hand, it is reasonable to assume that T. balaenophila occurs in the wide latitudinal interval between Chile and western Mexico and that lack of additional records of this species is due to lack of sampling. As a matter of fact, an additional, unpublished record of T balaenophila is available for a second Chilean locality: off Coquimbo, 30.3815 °S, 71.9626 °W, in 140 m depth (Bavarian State Collection of Zoology, Munich, Germany, Mol 20130032, 5 specimens; Enrico Schwabe, pers. comm., April 2015: coll. J. Sellanes). According to data available for this area (Helly & Levin 2004), this material was obtained close to the lower boundary of the OMZ.
Additional comments
The subgenus Tripoplax was originally proposed in 1919 (Berry 1919) within the genus Ischnochiton Gray, 1847. Tripoplax and Lepidozona have been considered as subgenera of Lepidozona Pilsbry, 1892 (Kaas & Van Belle 1987; Clark 1991, 2000; Eernisse 1998). Tripoplax has also been considered a natural assemblage based of its morphology and biogeography and elevated to genus (Clark 2008). An expanded definition of Tripoplax (Clark 2008) clearly stated that “insertion plates of intermediate valves with two to four slits” are characteristic of the genus. Tripoplax balaenophila was originally described in the genus Lepidozona (Schwabe & Sellanes 2004), with a slit formula of 1–2 for intermediate valves. In 2010, however, it was transferred to Tripoplax (as a new combination), following a recent redefinition of this genus (Clark 2008), without further comments on the slit formula (Schwabe & Sellanes 2010). This discrepancy in slit number and the assignment of “balaenophila” (with 1–2 slits on intermediate valves) to Tripoplax (with 2–4 slits on intermediate valves) cannot be solved without a more thorough review of both Lepidozona and Tripoplax. Tripoplax species are generally found in higher latitudes in the northern Pacific (Clark 2008), which is the case of the material examined herein (27°08′11″N). The presence of T. balaenophila in deep water from off both western Mexico (northern hemisphere) and Chile (southern hemisphere), however, is an interesting issue that needs to be addressed within a general biogeographically context of the distribution of the genera Tripoplax (southeastern and northern Pacific) and Lepidozona (with a worldwide distribution) in the eastern Pacific (Clark 2008; Schwabe & Sellanes 2010).
