Fig. 1
From: Insights on the origin of invasive copepods colonizing Basque estuaries; a DNA barcoding approach
Pseudodiaptomus marinus. a Maximum likelihood (ML) tree constructed with the HKY + G + I model of evolution. Forty MT-CO1 sequences (572 bp) from 8 different Pseudodiaptomus species were included in the analysis. The Bilbao estuary sequences (P. marinus AG39 – P. marinus AG42) clustered together in the lower branch (full black circle) along with the GenBank sole P. marinus record (grey circle). The specimen signalled by an asterisk that clustered within P. koreanus ones was identified as a new species (Pseudodiaptomus cf. inopinus Kyushu) in the original GenBank record (Accession no. AB576159) and might represent a misidentification. The scale bar indicates substitutions per nucleotide position. b Median-Joining (MJ) network analysis for the five P. marinus MT-CO1 sequences. Numbers correspond to the location of variable sites along the assembled region; white rhombuses represent missed haplotypes. Each circle represents one MT-CO1 haplotype; the size of the circles corresponds to the number of individuals sharing a haplotype (one except for a sole case with two individuals)